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. Author manuscript; available in PMC: 2020 Nov 7.
Published in final edited form as: Mol Cell. 2019 Sep 10;76(3):453–472.e8. doi: 10.1016/j.molcel.2019.07.036

Figure 5: MYOD expression is necessary for the maintenance of MYOD-regulated chromatin interactions. See alsoFigures S6 andS7.

Figure 5:

A, Scheme of the experimental approach used for all experiment in Fig. 5, EMPTY or MYOD IMR90 were exposed to doxycycline for 24h in GM followed by additional 48h of with/out doxycycline (ON/ON, ON/OFF).

B, Relative mRNA expression of Myod1 compared to EMPTY ON/ON (n=3). Data is represented as mean +/− SEM.

C, Immunoblot analysis of the whole cell lysate. GAPDH is used as loading control

D, Relative mRNA expression of TNNT2 compared to EMPTY ON/ON (n=3). Data is represented as mean +/− SEM

E, ChIP-qPCR for MYOD at TNNT2 promoter relative to EMPTY ON/ON (n=3).

F, Relative crosslink frequency (RCF) values between MYOD peak at TNNT2 promoter (view point – red eye – see Fig. 3H) and the enhancer. Data is represented as mean + SEM (n=3).

G, Relative mRNA expression of ITGA7 and RDH5 compared to EMPTY ON/ON (n=3). Data is represented as mean +/− SEM

H, ChIPqPCR for CTCF and MYOD at regulatory elements in the locus relative to EMPTY ON/ON (n=3).

I, Relative crosslink frequency (RCF) values between CTCF MYOD peak at ITGA7 promoter (view point – red eye – see Fig. 4L) and the CTCF MYOD peak in RDH5. Data is represented as mean + SEM (n=3).

T-test was used for statistical analysis, * p<0.05, ** p<0.01, *** p<0.001