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F-actin arrangement depends on NHE1 expression. Regardless of whether MV3 cells were seeded on glass (a, b), collagen type I (c, d) or a glutaraldehyde-fixed collagen type I substrate (e, f), NHE1 overexpression (b, d, f) is accompanied by a rearrangement of F-actin. In addition, the cells surface is characterized by the occurrence of numerous, actin-containing spike-like structures. Actin was labelled with phalloidin. Scale bar: 20 µm
