Skip to main content
. 2019 Oct 21;116(45):22635–22644. doi: 10.1073/pnas.1911362116

Fig. 4.

Fig. 4.

IFN response pathways in early pregnancy. (A) FPKM values of the IFN-related genes: type I and type II IFN receptors (IFNAR1, IFNAR2, IFNGR1, and IFNGR2), type I IFN (IFNA1 and IFNB1), and IFN-stimulated genes (ISG15 and ISG20). (B) Immunofluorescent localization of proteins implicated in potential IFN responses in human embryos between D8 and D12 (n = 3). (Scale bar, 100 µm.) (C) Western blot analysis of IFNAR2 and ISG15 (n = 3) and (D) phosphorylation of STAT1 (n = 3), mTOR (n = 3), AKT (n = 3), and MAPK1/3 (n = 3) in human embryos between D8 and D12. The expression of protein levels was normalized to ACTB (actin beta). The ratio of phosphorylated (p) and total protein abundance was used to determine the phosphorylation level of target proteins. All data are presented as the mean ± SEM.