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. 2019 Oct 7;116(45):22609–22618. doi: 10.1073/pnas.1903765116

Fig. 2.

Fig. 2.

The 5-Azacitidine treatment of NSCLC cell lines significantly down-regulates DNA repair associated genes. (A) Normalized enrichment score plot for REACTOME DNA Repair Pathway derived from preranked GSEA of relative RNA expression in H460 and A549 NSCLC cell lines. (B) Unsupervised hierarchical clustering by Euclidean distance of Z score distribution for relative RNA expression 5-AZA treated over Mock for REACTOME DNA Repair Pathway associated genes in H460, A549, H23, and H1299 NSCLC cell lines. Full gene list with associated Z score is available in SI Appendix, Table S2A. (C) Unsupervised hierarchical clustering by Euclidean distance of Z score distribution for relative RNA expression 5-AZA treated over Mock for Fanconi Anemia Pathway associated genes in H460, A549, H23, and H1299 NSCLC cell lines. Full gene list with associated Z score is available in SI Appendix, Table S2B. (D) Normalized enrichment score plots for HRD gene set derived from preranked GSEA of relative RNA expression in H460 and A549 NSCLC cell lines. (E) Relative RNA expression plot for select DNA repair genes (n = 9 from 3 experimental replicates performed in triplicate). Negative control c-MYC and positive controls B2M, IFI27, and OASL have previously been validated in Topper et al. (53). Above microarray data are derived from reanalysis of publicly deposited data GEO accession no. GSE104244 for NSCLC cell lines (A549, H23, H441, H460, H838, H1299, H1650, H1703, H1792, H1975, and H2170) treated with 500 nM 5-AZA as detailed in Topper et al. (53).