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. 2019 Jun 3;68(4):453–463. doi: 10.1538/expanim.18-0178

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©2019 Japanese Association for Laboratory Animal Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 1. — Characterization of bone marrow mesenchymal stem cells (BMMSCs) and osthole-treated-BMMSCs (OBMMSCs). (A) The analysis of surface antigens of BMMSCs and OBMMSCs by flow cytometry. Adipogenic (B, D) and osteogenic (C, E) differentiation of BMMSCs and OBMMSCs were determined by Oil Red O staining and Alizarin Red S staining respectively. (F) Proliferation activity of BMMSCs and OBMMSCs. Data were analyzed by Student’s t-test. Results are presented as means ± SD, *P<0.05.