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. 2019 Jun 3;68(4):453–463. doi: 10.1538/expanim.18-0178

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©2019 Japanese Association for Laboratory Animal Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 4. — Autophagy level in bone marrow mesenchymal stem cells (BMMSCs) and osthole-treated-BMMSCs (OBMMSCs). (A, B) Western blot was performed to examine expression of Beclin1 and LC3 in the ovariectomy (OVX) and Sham group at protein level. (E) Real-time PCR was performed to detect the mRNA expression of Beclin1 and LC3. (C, D, F) Expression of Beclin1 and LC3 in BMMSCs and OBMMSCs at protein and mRNA level was also observed. Data were analyzed by Student’s t-test. Results are presented as means ± SD, *P<0.05.