Figure 1.

Severe ischemic stroke in C57BL/6J mice does not cause spontaneous pneumonia. Brain, spleen, and lung tissues were analyzed 24 and 72 hours following tMCAO or sham operation. A, Representative images showing ipsilateral brain infarcts following tMCAO but not sham operation by TTC staining. B‐D, Percentage of infarcts within the ipsilateral hemisphere (B), cortex (C), and corpus striatum (D) following tMCAO (filled circle) or sham controls (open circle) quantified by Image J. E, Neurological deficit scores of the mice 24 and 72 hours following tMCAO (filled circle) or sham controls (open circle). See Section 2 for score definition. F, Cell number from the spleens of mice 72 hours following tMCAO (filled circle) or sham controls (open circle). G, Representative images from H&E staining of lung tissues 72 hours following tMCAO (right) or sham operation (left). Images from all animals are shown in Figure S1. Data shown are combined results from two independent experiments with n = 6 animals per group (sham 24 hours, tMCAO 24 hours, sham 72 hours, and tMCAO 72 hours). ***P < .001. H&E, hematoxylin and eosin; tMCAO, transient middle cerebral artery occlusion; TTC, Triphenyltetrazolium chloride