Figure 5.

Ischemic stroke leads to a significant loss of lymphocytes in the lungs independent of apoptosis. A, Representative plots showing the identification of lymphocytes in the lungs by surface markers listed on Table 1. CD4+ (L10) and CD8+ (L11) T cells were first gated on CD45+ B220− CD11c− cells shown in Figure 3H. NK cells (L12) and NKT cells (L13) were gated on CD4− CD8− cells (*), then further gated on NK1.1+ cells (**). Representative plots for the identification of B cells shown in Figure 3H. B‐F, Graphs showing percentage of CD4+ T cells (B), CD8+ T cells (C), B cells (D), NK cells (E), and NKT cells (F) of individual animals 24 and 72 hours following tMCAO (filled circle) or sham operation (open circle). G‐H, Graphs showing percentage of annexin‐V+ CD4+ T cells (G), CD8+ T cells (H), B cells (I), and NK cells (J) 24 and 72 hours following tMCAO (filled bar) or sham operation (open bar). Data shown are combined results from three independent experiments with n = 12 animals per group (sham 24 hours, tMCAO 24 hours, sham 72 hours, tMCAO 72 hours). *P < .05; ***P < .001. NKT, natural killer T; NS, not statistically different; tMCAO, transient middle cerebral artery occlusion