Figure 6.

Ischemic stroke does not induce the activation of caspase 3 in the lungs. Spleen and lung tissues were dissected 72 hours following tMCAO or sham operation. The cleaved (activated) form of caspase 3 was measured by immunohistochemistry assay. Shown are representative images with n = 6 per group for the lung tissues and n = 3 per group for the spleens. Spleen samples following tMCAO serve as positive control. Brown color indicates positive signal. The tissues were counterstained with hematoxylin. Lung images from all animals are shown in Figure S2. tMCAO, transient middle cerebral artery occlusion