Fig. 1.

High-molecular-weight kininogen (HK) levels before and after induction of pneumonia-derived sepsis in mice pretreated with control (Ctrl) antisense oligonucleotide (ASO) or kininogen (KNG) ASO. Target gene (Kng1) expression level indicated as its mRNA by quantitative RT-PCR (A) and plasma HK level by Western blot after SDS-PAGE under reducing conditions (B); plasma HK levels before and during Klebsiella pneumonia in mice treated with Ctrl ASO (n = 8) or KNG ASO (n = 8) (D and E). Quantification of HK levels in naïve and infected mice (C) determined by densitometry of the HK bands at 120 kDa in B, D, and E and compared with a serial dilution of plasma as shown in B. Serial data were analyzed by Kruskal-Wallis test followed by Mann-Whitney U-tests. ^ΦP < 0.05 vs. corresponding time (t) = 0 group; *P < 0.05 and ***P < 0.001, Mann-Whitney U-tests between KNG ASO vs. Ctrl ASO.