Fig. 1.

Effects of the acetaldehyde-generating system (AGS) on expression of hepatitis C virus (HCV) RNA and miRNAs. HCV-infected CYP2E1+ Huh7.5 (RLW) cells were treated with AGS for the indicated time. A: RT-PCR analysis of HCV RNA in RLW cells. GAPDH mRNA was used to normalize the gene of interest. B and C: quantitative PCR analysis of miR-122 and miR-34a. Z30 RNA was used to normalize the gene of interest. All data (representative results and quantification) were generated from three independent experiments and presented as means ± SE. Bars with different letters are significantly different at P ≤ 0.05.