Fig. 4.

Ach-induced apoptosis is attenuated by miR-34a antagonist in HCV-infected RLW cells. Immunoblotting of cleaved caspase-3 and β-actin (A) and quantification of Western blot (B). HCV-infected RLW cells were transfected using RNAiMAX transfection reagent (Life Technologies) with miR-34a antagonist, LNA-34a, an antisense-locked nucleic acid oligonucleotide specific for human miR-34a (MIMAT0000255) and LNA-negative control A. LNAs were obtained from Exiqon (Woburn, MA) and used at a final concentration of 20 nM. Twenty-four hours after transfection, cells were exposed to AGS for 48 h. Caspase-3 cleavage was detected by Western blot analysis. All data (representative results and quantification) were generated from three independent experiments. Data are presented as means ± SE. Bars with different letters are significantly different at P ≤ 0.05.