Fig. 5.

Ach regulates HCV RNA levels and induces apoptosis in HCV-infected hepatocytes. A: HCV-RNA in hepatocytes treated with AGS for 24 h and 48 h, RT-PCR. B and C: immunoblotting of cleaved caspase-3, β-actin, and quantification. Human primary hepatocytes were infected with JFH-1 virus for 72 h and then treated with AGS for 24 h and 48 h. D and E: immunoblotting of Cleaved PARP, total PARP, and quantification. All data (representative results and quantification) were generated from three independent experiments. Data are presented as means ± SE. Bars with different letters are significantly different at P ≤ 0.05.