Fig. 2.

Renal expression of acute inflammation-related genes [chemokine (C-C motif) ligand 2 (CCL2), IL-6, matrix metalloproteinase (MMP)-9, and plasminogen activator inhibitor 1 (PAI-1)] in aged male mice with targeted disruption of the heme oxygenase (HO)-2 gene (HO-2^−/− mice) and littermate control (HO-2^+/+) mice on day 2 after acute (15 min) renal ischemia (IR). mRNA expression of CCL2 (A), IL-6 (B), MMP-9 (C), and PAI-1 (D) was determined by quantitative real-time RT-PCR. Values are means ± SE; n = 4 mice subjected to sham operation and n = 11 HO-2^+/+ and 12 HO-2^−/− mice subjected to IR. *P < 0.05 vs. HO-2^+/+ mice subjected to IR.