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. 2019 Jun 5;317(2):L283–L294. doi: 10.1152/ajplung.00337.2018

Fig. 1.

Fig. 1.

Three-dimensional culture system with alveolar type II cells and fibroblasts. A: scheme of the culture system. The epithelial cells and the fibroblasts are cultured by themselves or together. The culture system is a floating gel composed of collagen and Matrigel, and the cultures can be under air-liquid or submerged conditions. The fibroblasts and the epithelial cells are reisolated at the end of the experiment for assays of gene expression under submerged conditions. B: hematoxylin and eosin-stained image of the floating coculture system. Cuboidal epithelial cells are on the top of the gel and fibroblasts within the gel. The alveolar type II cells in the cocultures are highly polarized. C: the red apical staining is HTII-280, the green basolateral marker is E-cadherin, and the nuclei are blue with DAPI. D: the red apical staining is Muc1, and the basolateral green staining is E-cadherin. E: the red apical staining is Muc1, and the green staining is receptor for advanced glycation end products. F: the red apical staining is Muc1, and the green staining is proSP-B.