Fig. 4.

Inhibition of type 1 collagen (COL1A1), smooth muscle actin (ACTA2), hepatocyte growth factor (HGF), fibroblast growth factor 7 (FGF7), and fibroblast growth factor 10 (FGF10) by the epithelial cells was not blocked by cyclooxygenase 1 (COX1) and COX2 inhibitors. The epithelial cells and the fibroblasts were cultured alone or together for 8 days and then processed as described for Fig. 2. Indomethacin, DMSO, NS-398, and transforming growth factor-β were added 4 days before harvest. There were five individual experiments with different epithelial and fibroblasts cells for the comparisons of COL1A1, ACTA2, and FN1 and four for HGF, FGF7, and FGF10. *P < 0.05 by analysis of variance to assess if epithelial cells inhibited fibroblast gene expression and if they persisted in the presence of indomethacin (columns 1, 5, and 6). The panels are as follows: A: COL1A1; B: ACTA2; C: FN1; D: HGF; E: FGF7; and F: FGF10.