Fig. 3.

Myeloid-derived suppressor cells (MDSCs) cause pulmonary vascular remodeling. A: percentage (%) of arginase 1 (Arg1) and inducible nitric oxide synthase (iNOS)-positive granulocytic (PMN-MDSCs) in MDSCs versus neutrophil (PMN)-treated wild-type (WT; C57BL/6J) vehicle (Veh) and bleomycin (Bleo) mice. B: complete:partial muscularized pulmonary vessel ratio in treated groups. C: representative α-smooth muscle actin (α-SMA; brown)-stained pulmonary vessels in treated groups, at low magnification (×20; scale bar 100 μm). D: right ventricular systolic pressure (RVSP) in IgG or anti-Gr1 (RB6-C85), MDSC inhibited, WT mice exposed to Veh or Bleo. n = 3–6 Mice/group. All data are presented as means ± SE. Student’s t test was used for single comparisons. P < 0.05 was considered significant.