Table 1.

Primer sequences for RT-PCR using SYBR Green

Gene Symbol	Forward (5′–3′)	Reverse (5′–3′)	Amplicon Size, bp
Bmal1	TGGAGGGACTCCAGACATTC	TTGCTGCCTCATCGTTACTG	173
Per1	GTCCCCTGGTCCTCTACACA	GCCCGAGATTCAATGAAGAG	159
Per2	AGCCACCCTGAAAAGGAAGT	GGTGAGGGACACCACACTCT	184
Per3	GTCGAGAGGAGGTGCTGAAG	TCTGTCTTCACAGGCGACAC	173
Rev-Erbα	GGCACCTGCCAACAGTCTA	GCTGAGAAAGGTCACGGAAG	197
Rora	GGAAGAGTTTGTGTTCTATGCACC	TTCCATCTTCTCGGTGGTTC	177
Cry1	TTCACTGCTACTGCCCTGTG	CACTTGGCAACCTTCTGGAT	151
Myod	TACCCAAGGTGGAGATCCTG	CATCATGCCATCAGAGCAGT	200
Dbp	TCTAGGGACACACCCAGTCC	TGGTTGAGGCTTCAGTTCCT	159
Rplp0	CAACCCAGCTCTGGAGAAAC	GTTCTGAGCTGGCACAGTGA	169
Drp1	AGGAACCAACAACAGGCAAC	TCACAATCTCGCTGTTCTCG	190
Fis1	AAGTATGTGCGAGGGCTGTT	ACAGCCAGTCCAATGAGTCC	167
Opa1	GATGACACGCTCTCCAGTGA	TCGGGGCTAACAGTACAACC	177
Mfn1	GCTGTCAGAGCCCATCTTTC	CAGCCCACTGTTTTCCAAAT	195
Mfn2	AGCGCCAGTTTGTGGAATAC	CTTTCTTGTTCATGGCAGCA	177
Ppargc1a	AAGACGGATTGCCCTCATTT	AGTGCTAAGACCGCTGCATT	191
Sirt1	AGTTCCAGCCGTCTCTGTGT	CTCCACGAACAGCTTCACAA	198
Gapdh	GTTGTCTCCTGCGACTTCA	TGCTGTAGCCGTATTCATTG	124
Id1	TACGACATGAACGGCTGCTA	GTGGTCCCGACTTCAGACTC	155

Bmal1, brain and muscle aryl hydrocarbon receptor nuclear translocator (ARNT)-like 1; bp, base pair; Cry1, cryptochrome circadian regulator 1; Dbp, D-box-binding proline- and acid-rich basic region leucine zipper (PAR bZIP) transcription factor; Drp1, dynamin-1-like protein; Fis1, mitochondrial fission 1 protein; Id1, inhibitor of DNA binding 1, helix-loop-helix (HLH) protein; Mfn1 and Mfn2, mitofusin-1 and -2, respectively; Myod, myogenic differentiation; Opa1, dynamin-like 120-kDa protein, mitochondrial; Per1–3, period circadian regulator 1–3, respectively; Ppargc1a, peroxisome proliferator-activated receptor-γ coactivator 1α; Rev-Erbα, nuclear receptor subfamily 1 group D member 1α; Rora, retinoic acid receptor (RAR)-related orphan receptor-α; Rplp0, ribosomal protein lateral stalk subunit P0; Sirt1, sirtuin 1.