FIGURE 3.

Glycogen content dictates latency to CAP failure in the MON model. (A) In MONs pre-incubated in 10 mM glucose (straight line), the CAP starts to fail at about 20 min after introducing 0 mM glucose aCSF, i.e., simulated aglycaemia, and falls rapidly to zero in the continued presence of aglycemia. In nerves pre-incubated for 2 h in increasing concentrations of glucose (circle – 15 mM, triangle – 20 mM, square – 30 mM), the latency to CAP failure increased in line with the glucose concentration. (B) There is a linear relationship between glycogen content (pmole μg protein^–1) at the onset of aglycemia and latency to CAP failure.