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. 2019 Oct 23;7:245. doi: 10.3389/fcell.2019.00245

FIGURE 5.

FIGURE 5

Brg1 is essential for NOX4 expression in vivo and in vitro. (A,B) WT and ecKO mice were subjected to BDL. NOX4 expression was examined by qPCR and Western. N = 6 mice for each group. (C) Primary LSECs were isolated from WT and ecKO mice subjected to BDL. NOX4 expression was examined by qPCR. N = 3 mice for each group. (D,E) EAhy926 cells were transfected with WT or ED Brg1 followed by treatment with TGF-β. NOX4 expression was examined by qPCR and Western. (F,G) EAhy926 cells were transfected with siRNA targeting Brg1 or SCR followed by treatment with TGF-β. NOX4 expression was examined by qPCR and Western. (H,I) EAhy926 cells were treated with TGF-β in the presence or absence of PFI-3. NOX4 expression was examined by qPCR and Western. N = 3 for all the in vitro experiments. ∗p < 0.05.