FIGURE 6.

Brg1 activates NOX4 transcription by interacting with AP-1 and SMAD. (A) Different NOX4 promoter-luciferase constructs were transfected into EAhy926 cells with or without Brg1 followed by treatment with TGF-β. Luciferase activities were normalized by both protein concentration and GFP fluorescence. (B) Wild type (WT) or mutant (MT) NOX4 promoter-luciferase constructs were transfected into EAhy926 cells with or without Brg1 followed by treatment with TGF-β. Luciferase activities were normalized by both protein concentration and GFP fluorescence. (C) EAhy926 cells were treated with TGF-β and harvested at indicated time points. ChIP assays were performed with anti-Brg1. (D,E) EAhy926 cells were transfected with indicated siRNAs followed by treatment with TGF-β. Knockdown efficiencies were examined by Western. ChIP assays were performed with anti-BRG1. (F) EAhy926 cells were treated with or without TGF-β for 48 h. Re-ChIP assays were performed with indicated antibodies. N = 3 for all the in vitro experiments. ^∗p < 0.05.