FIGURE 7.

Brg1 regulates NOX4 transcription by recruiting histone modifying enzymes. (A–F) EAhy926 cells were transfected with siRNA targeting Brg1 or SCR followed by treatment with TGF-β. ChIP assays were performed with indicated antibodies. (G,H) EAhy926 cells were transfected with siRNA targeting JMJD2B or SCR followed by treatment with TGF-β. NOX4 expression was examined by qPCR and Western. (I,J) EAhy926 cells were transfected with siRNA targeting BRG1 or SCR followed by treatment with TGF-β in the presence or absence of NOX4. ROS levels were examined by luminescence assay. Gene expression levels were examined by qPCR. N = 3 for all the in vitro experiments. (K) A schematic model. ^∗p < 0.05.