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. 2019 Oct 22;10:2476. doi: 10.3389/fimmu.2019.02476

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Copyright © 2019 van Eijk, Hillaire, Rimmelzwaan, Rynkiewicz, White, Hartshorn, Hessing, Koolmees, Tersteeg, van Es, Meijerhof, Huckriede and Haagsman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of iSP-D and comparison with other SP-Ds. (A) Reducing (left) and non-reducing (right) SDS-PAGE (12%) and Coomassie staining of purified full-length SP-Ds. (B) Western blot analysis of reduced and non-reduced hSP-D and iSP-D, followed by immunodetection with rabbit anti-human SP-D antibodies and goat anti-rabbit HRPO conjugate. The different bands visible in the non-reducing lanes are due to the presence of various oligomeric forms of SP-D that typically occurs upon non-reducing SDS-PAGE analysis of fully assembled SP-D. (C) Analysis of SP-Ds by Atomic Force Microscopy in ambient conditions after deposition on mica and scanning in tapping mode. The high points are colored lighter and lower points darker—full scale, black to white is 2 nm.