Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 22;10:2476. doi: 10.3389/fimmu.2019.02476

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 van Eijk, Hillaire, Rimmelzwaan, Rynkiewicz, White, Hartshorn, Hessing, Koolmees, Tersteeg, van Es, Meijerhof, Huckriede and Haagsman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Effect of SP-D assembly on HAI activity of IAV. The inhibitory activity of iSP-D, pSP-D and hSP-D was measured for oligomeric structures and for trimeric subunit structures of SP-D. Increasing concentrations (highest concentration tested was 2,000 ng/ml) of SP-D were mixed with 2 HAU of IAV (A/PuertoRico/8/34 (H1N1) strain) and after 1 h erythrocytes were added. After 3 h incubation at room temperature, IAV-induced hemagglutination patterns revealed the lowest concentration of SP-D required to fully inhibit hemagglutination by IAV. The assay was performed in PBS containing 1 mM of CaCl₂ and 0.5 mM of MgCl₂. Averages are based upon five independent experiments.