Table 1.
Comparison of saccharide selectivity of recombinant pSP-D, hSP-D, and iSP-D.
| Sugar inhibitor | pSP-D | hSP-D | iSP-D |
|---|---|---|---|
| Myo-Inositol | 0.9 (0.4) | 5.6 (0.9) | 17.3 (2.1) |
| Maltose | 2.0 (0.2) | 3.3 (0.5) | 4.1 (0.1) |
| L-Fucose | 2.2 (0.8) | 3.8 (0.4) | 4.3 (0.6) |
| Fructose | 2.8 (1.1) | 3.1 (0.1) | 4.8 (1.3) |
| D-Glucose | 2.8 (0.8) | 4.6 (1.5) | 7.8 (1.4) |
| D-Mannose | 4.6 (1.3) | 3.6 (0.5) | 5.3 (2.1) |
| D-Galactose | 6.5 (0.8) | 13.5 (4.8) | 13.6 (3.4) |
| ManNAc | 8.0 (0.1) | 2.2 (0.2) | 3.1 (0.7) |
Microtiter wells were coated with BSA-mannan and were incubated with SP-Ds in the presence of increasing concentrations of sugars listed. Binding of collectin to BSA-mannan was measured by immunodetection as described in the Materials and Methods section. Results are expressed as concentrations (mM) of saccharide required for 50% inhibition of SP-D binding (IC50) to BSA-mannan. Data are averages of three experiments; SD values in parentheses.