Table 2.
Hemagglutination inhibition of IAV by SP-Ds.
| H1N1 influenza A viruses | SP-D preparation | H3N2 influenza A viruses | SP-D preparation | ||||
|---|---|---|---|---|---|---|---|
| pSP-D | hSP-D | iSP-D | pSP-D | hSP-D | iSP-D | ||
| Swine (pandemic 2009) | Swine | ||||||
| A/Netherlands/602/09 | 167 (72) | >1,000 (0) | 458 (473) | A/swine/Oedenrode/7C/96 | 63 (0) | >1,000 (0) | 104* (36) |
| A/Califomia/4/09 | 161 (94) | >1,000 (0) | 479 (452) | A/swine/Netherlands/849/93 | 16 (0) | >1,000 (0) | 8* (0) |
| Swine (classical) | A/swine/Utrecht/4/85 | 16 (0) | >1,000 (0) | 16* (0) | |||
| A/swine/Shope/11/56 | 458 (72) | >1,000 (0) | 583 (382) | A/swine/Ukkel/1/84 | 44 (16) | >1,000 (0) | 57* (9) |
| A/swine/Iowa/15/30 | 750 (354) | >1,000 (0) | >1,000 (0) | Human | |||
| A/NewJersey/8/76 | 60 (5) | >1,000 (0) | 83* (36) | A/Netherlands/348/07 | 2 (2) | 4 (3) | 2 (2) |
| Swine (avian-like) | A/Netherlands/548/05 | 1 (1) | 4 (0) | 1* (1) | |||
| A/swine/Netherlands/25/80 | 188 (108) | >1,000 (0) | 375* (217) | A/Netherlands/312/03 | 1 (0) | 5 (3) | 1 (0) |
| A/swine/Netherlands/1/87 | 208 (72) | >1,000 (0) | 229* (95) | A/Netherlands/35/93 | 16 (0) | 833 (289) | 52* (63) |
| Human | Avian | ||||||
| A/Netherlands/384/06 | 9 (2) | 89 (33) | 17 (10) | A/mallard/Netherlands/19/05 | 26 (9) | >1,000 (0) | 52* (18) |
| A/Netherlands/246/08 | 9 (6) | 34 (25) | 11 (4) | A/mallard/Netherlands/51/08 | 12 (7) | 688 (541) | 16 (8) |
| A/PuertoRico/8/34 | 313 (165) | >1,000 (0) | 313* (165) | A/mallard/Sweden/57/03 | 11 (17) | >1,000 (0) | 26* (9) |
| A/Netherlands/26/07 | 36 (36) | 83 (36) | 21* (16) | A/mallard/Netherlands/1/07 | 18 (12) | 385 (532) | 10 (4) |
| A/USSR/90/77 | 4 (4) | 3 (1) | 3 (4) | ||||
| Avian | H5N1 influenza A viruses (n = 1) | ||||||
| A/mallard/Netherlands/15/05 | 146 (95) | >1,000 (0) | 208* (72) | A/HongKong/156/97 (clade 0) | 500 | >1,000 | >1,000 |
| A/mallard/Netherlands/24/06 | 45 (30) | 681 (553) | 25 (11) | A/Vietnam/1194/0 (clade 1) | 250 | >1,000 | >1,000 |
| A/white fronted goose/Netherlands/1/07 | 104 (36) | >1,000 (0) | 125* (0) | A/lndonesia/5/05 (clade 2.1) | 250 | >1,000 | >1,000 |
| A/common teal/Netherlands/10/00 | 83 (36) | >1,000 (0) | 120* (113) | ||||
Shown is the inhibitory activity of pSP-D, hSP-D, and iSP-D against a broad panel of IAVs isolated from different animal species (swine, human, avian) and different subtypes (H1N1, H3N2, and H5N1). Values are lowest concentrations (ng/ml) required to fully inhibit 20 hemagglutination units/ml of the respective viruses diluted in PBS. A value of “>1,000” indicates no inhibition at the highest SP-D concentrations tested. Assay procedure and details are described in Materials and Methods section. Measurements were performed in duplo (except for H5N1 viruses: n = 1) and shown is the mean of three independent experiments with standard deviations in parentheses. Statistical analysis by two-tailed paired Student's t-test (*P < 0.05 vs. hSP-D).