Figure 5.

Reduced expression of activation markers in RelA-deficient Tregs at steady state. Analyses in the indicated organs (thy, thymus; spl, spleen; liv, liver) of 8 week-old Foxp3^Cre/wt (Cre/wt – gray bars) and Foxp3^Cre/wt Rela^lox (Cre/wt Rela^lox–green bars) mice. (A) Representative density plots among CD4⁺ cells to define Tregs expressing CRE (CRE⁺) and percentages of CRE⁺ among total Tregs in sdLN. Representative density plots and proportions of CD44^hi CD62L^low (B), Ki67⁺ (C), CD103⁺ (D) and MFI of GITR (E) among CRE⁺ Tregs of sdLN. Bars show the means of pooled independent experiments and error bars represent SEM. For mouse and experiment numbers, see Supplementary Table 1. The two-tailed unpaired nonparametric Mann–Whitney U-test was used. *p < 0.05, **p < 0.01, ***p < 0.001.