TABLE 1.

Oligonucleotide sequences used for quantitative PCR (qPCR) approaches.

Target	Primer (reference)	DNA sequence (bp)	Annealing temperature (°C)
mcrA gene	mlas-mod (Angel et al., 2011)	5′-GGYGGTGTMGGDTTCACMCARTA-3′	57
	mcrA-rev (Angel et al., 2011)	5′-CGTTCATBGCGTAGTTVGGRTAGT-3′
fhs gene	fhs-f (Xu et al., 2009)	5′-gTWTgggAAAAggYggMgAAgg-3′	55
	fhs-r (Xu et al., 2009)	5′-gTATTgDgTYTTRgCCATACA-3′
Bacteria	519f (Imachi et al., 2008)	5′-CAGCMGCCGCGGTAANWC-3′	50
	907r (Imachi et al., 2008)	5′-CCGTCAATTCMTTTRAGTTT-3′