Fig. 2.

Extracellular (e)ATP induces neutrophil migration and promotes inflammation. A: C57B/6 mice were injected with ATP-γ-S (4 mg/kg ip). Peritoneal exudate cells (PECs) were harvested 3 h later, stained with Ly6G and assayed by flow cytometry. B: quantification of flow data. eATP was able to elevate plasma interleukin-6 (IL-6; C) and plasma tumor necrosis factor-α (TNF-α; D) levels. Effect of ATP-γ-S (4 mg/kg ip) addition to caerulein injection on plasma IL-6 (E) and plasma TNFα levels (F). ATP was injected after 7th injection of caerulein, and mice were euthanized 1 h after ATP injection. *P < 0.05; n = 6 in each group.