Fig. 7.

Effect of extracellular (e)ATP on mouse acinar cells. A: no changes in intracellular calcium levels after eATP (100 μM) treatment. B: list of most upregulated inflammatory genes vs. controls. PCR array was performed using SYBR Green dye according to manufacturer instructions. C: representative protein blot of p38 phosphorylation and IκBα degradation in response to eATP treatment. D: eATP treatment induced secretion of tumor necrosis factor-α (TNFα) in dose-dependent manner. E: secretion of leukotriene B4 (LTB4) in medium in response to ATP (100 μM) treatment; data were normalized to total protein content in each well. F: eATP treatment induces acinar cell death. %Lactate dehydrogenase (LDH) activity was measured in medium supernatant and normalized by total protein content. Fresh acinar cells were incubated with ATP for 3 h and TNF-α, LTB4, and LDH activities were measured in supernatant. *P <0.05; n=5–6 in each group.