Fig. 1.

Characterization and validation of inducible conditional (intestine-specific) sodium-dependent multivitamin transporter (SMVT)-inducible, conditional knockout (icKO) mice. A: Kaplan-Meier survival curve for intestine-specific SMVT-icKO mice (n = 8). SMVT-icKO (labeled as red dotted line) mice die between days 5 and 9 posttamoxifen treatment. B: expression of SMVT mRNA in small intestine (jejunum) (i), large intestine (cecum) (ii), kidney (iii), and liver (iv) of SMVT-icKO mice and their sex-matched control littermates [n = 5 in each group; **P < 0.01 and not significant (NS)]. C: Western blot analysis of SMVT protein in small intestine (jejunum) of SMVT-icKO mice and their control littermates (n = 4 in each group; *P < 0.05). D: carrier-mediated [³H]biotin uptake in vivo using intact jejunal loops of SMVT-icKO mice and their sex-matched control littermates (n = 5 in each group; **P < 0.01). Data are presented as box and dot-whisker plots, and statistical significance is analyzed by Mann-Whitney test.