Fig. 7.

Gravin-t/t mice show decreased activation of sterol regulatory element binding protein (SREBP)-2 in response to high-fat diet (HFD). mRNA expression of the cholesterol metabolism genes SREBP-1c (A), SREBP-2 (B), and SREBP-2n (C) were quantified in the liver using reverse transcriptase quantitative PCR. Expression of SREBP-2 (B) was significantly decreased in gravin-t/t normal diet (ND)-fed versus wild-type (WT) ND-fed mice, whereas expression of SREBP-2n, the active form of SREBP-2 found in liver homogenized lysates, was significantly decreased in both gravin-t/t ND-fed and gravin-t/t HFD-fed mice. Results are presented as the mean ± SE. n = 6 (A–C); *P < 0.05, **P < 0.01, and ***P < 0.0001. Comparisons between two groups were determined by an unpaired two-tailed Student’s t test, and comparisons between multiple groups were determined by one-way ANOVA followed by post hoc Tukey test. MM, molecular mass.