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. 2019 Aug 23;317(4):H793–H810. doi: 10.1152/ajpheart.00215.2019

Table 1.

List of the reverse transcriptase quantitative PCR primers

Gene (Primer) Sequence
3′ end of exon 3 of gravin
    Forward 5′-GCCAGTGAAGAACATGAGCA-3′
    Reverse 5′-TGCAATCTGCTTTGTCTTGG-3′
5′ end of exon 3 of gravin
    Forward 5′-GAGCAGGAGACCACCAAGAG-3′
    Reverse 5′-TTCTCCATCTTTGGCTGCTT-3′
FAS
    Forward 5′-GGAGGTGGTGATAGCCGGTAT-3′
    Reverse 5′-TGGGTAATCCATAGAGCCCAG-3′
LPL
    Forward 5′-TTGCCCTAAGGACCCCTGAA-3′
    Reverse 5′-ACAGAGTCTGCTAATCCAGGAAT-3′
SREBP-1c
    Forward 5′-GATGTGCGAACTGGACA-3′
    Reverse 5′-CATAGGGGGCGTCAAACAG-3′
HMGCR
    Forward 5′-GGCATTTGACAGCACTAGCA-3′
    Reverse 5′-CTTTGCATGCTCCTTGAACA-3′
LDLR
    Forward 5′-CTCGCTGGTGACTGAAAACA-3′
    Reverse 5′-CAAAGGAAGACGAGGAGCAC-3′
SREBP-2
    Forward 5′-AAGTCTGGCGTTCTGAGGAA-3′
    Reverse 5′-CACAAAGACGCTCAGGACAA-3′
Exon 1 of gravin
    Forward 5′-ATGGGTGCAGGCAGTTCC-3′
    Reverse 5′-CGGGATCTCCAGCTGCTC-3′
Exon 2 of gravin
    Forward 5′-CTCCCACAGAAGAATGGTCAG-3′
    Reverse 5′-GACTTCTTCCTCTTGCCCATC-3′
Exon 3A of gravin
    Forward 5′-GAGCAGGAGACCACCAAGAG-3′
    Reverse 5′-TTCTCCATCTTTGGCTGCTT-3′
Exon 3B of gravin
    Forward 5′-TGGGCATCCTTCAAAAAGATG-3′
    Reverse 5′-CCTTAAGCTCTTCTTCCTTGT-3′
Exon 3C of gravin
    Forward 5′-GCCAGTGAAGAACATGAGCA-3′
    Reverse 5′-TGCAATCTGCTTTGTCTTGG-3′
18s RNA
    Forward 5′-TCAAGAACGAAAGTCGGAGG-3′
    Reverse 5′-GGACATCTAAGGGCATCA C-3′

FAS, fatty acid synthase; HMGCR, 3-hydroxy-3-methyl-glutaryl-CoA reductase; LDLR, low-density lipoprotein receptor; LPL, lipoprotein lipase; SREBP, sterol regulatory element binding protein.