Table 1.
Gene (Primer) | Sequence |
---|---|
3′ end of exon 3 of gravin | |
Forward | 5′-GCCAGTGAAGAACATGAGCA-3′ |
Reverse | 5′-TGCAATCTGCTTTGTCTTGG-3′ |
5′ end of exon 3 of gravin | |
Forward | 5′-GAGCAGGAGACCACCAAGAG-3′ |
Reverse | 5′-TTCTCCATCTTTGGCTGCTT-3′ |
FAS | |
Forward | 5′-GGAGGTGGTGATAGCCGGTAT-3′ |
Reverse | 5′-TGGGTAATCCATAGAGCCCAG-3′ |
LPL | |
Forward | 5′-TTGCCCTAAGGACCCCTGAA-3′ |
Reverse | 5′-ACAGAGTCTGCTAATCCAGGAAT-3′ |
SREBP-1c | |
Forward | 5′-GATGTGCGAACTGGACA-3′ |
Reverse | 5′-CATAGGGGGCGTCAAACAG-3′ |
HMGCR | |
Forward | 5′-GGCATTTGACAGCACTAGCA-3′ |
Reverse | 5′-CTTTGCATGCTCCTTGAACA-3′ |
LDLR | |
Forward | 5′-CTCGCTGGTGACTGAAAACA-3′ |
Reverse | 5′-CAAAGGAAGACGAGGAGCAC-3′ |
SREBP-2 | |
Forward | 5′-AAGTCTGGCGTTCTGAGGAA-3′ |
Reverse | 5′-CACAAAGACGCTCAGGACAA-3′ |
Exon 1 of gravin | |
Forward | 5′-ATGGGTGCAGGCAGTTCC-3′ |
Reverse | 5′-CGGGATCTCCAGCTGCTC-3′ |
Exon 2 of gravin | |
Forward | 5′-CTCCCACAGAAGAATGGTCAG-3′ |
Reverse | 5′-GACTTCTTCCTCTTGCCCATC-3′ |
Exon 3A of gravin | |
Forward | 5′-GAGCAGGAGACCACCAAGAG-3′ |
Reverse | 5′-TTCTCCATCTTTGGCTGCTT-3′ |
Exon 3B of gravin | |
Forward | 5′-TGGGCATCCTTCAAAAAGATG-3′ |
Reverse | 5′-CCTTAAGCTCTTCTTCCTTGT-3′ |
Exon 3C of gravin | |
Forward | 5′-GCCAGTGAAGAACATGAGCA-3′ |
Reverse | 5′-TGCAATCTGCTTTGTCTTGG-3′ |
18s RNA | |
Forward | 5′-TCAAGAACGAAAGTCGGAGG-3′ |
Reverse | 5′-GGACATCTAAGGGCATCA C-3′ |
FAS, fatty acid synthase; HMGCR, 3-hydroxy-3-methyl-glutaryl-CoA reductase; LDLR, low-density lipoprotein receptor; LPL, lipoprotein lipase; SREBP, sterol regulatory element binding protein.