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. 2019 Aug 14;317(4):F1022–F1033. doi: 10.1152/ajprenal.00077.2019

Fig. 7.

Fig. 7.

Blockade of sonic hedgehog (Shh) signaling inhibits renal lymphangiogenesis and fibrosis after ischemia-reperfusion injury (IRI). A: inhibition of Shh signaling by cyclopamine (CPN) reduced renal lymphangiogenesis and fibrosis in vivo. Top: immunofluorescent staining showing that CPN inhibited lymphangiogenesis in the fibrotic kidneys 10 days after IRI. Bottom: Sirius red staining showing that CPN ameliorated renal fibrotic lesions 10 days after IRI. B: quantitative determination of lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1)+ vessels in the different groups. *P < 0.05 vs. sham controls; ††P < 0.1 vs. IRI + vehicle (Veh; n = 3–4). C: quantitative RT-PCR analyses showing that CPN inhibited renal expression of LYVE-1 mRNA after IRI injury. *P < 0.05 vs. sham controls (n = 4). D: quantitative determination of renal fibrotic lesions in the different groups. *P < 0.05 vs. sham controls; †P < 0.05 vs. IRI + Veh (n = 3–4).