Fig. 8.

Blockade of sonic hedgehog (Shh) signaling by cyclopamine (CPN) does not affect normal kidney morphology and function in mice. Mice were subjected to daily injection of CPN at 5 mg/kg body wt for 10 days. A–C: CPN did not affect the levels of serum creatinine (A), blood urea nitrogen (BUN) (B), and urinary albumin (C) in mice. No significant (n.s.) difference was found between vehicle and CPN-treated groups. D: representative micrographs showing the structure of major organs including the kidney, liver, heart, lung, and intestine in vehicle- or CPN-treated groups. Scale bar = 50 μm.