Fig. 5.

Principal component analysis (PCA) of kidney lysates in positive mode. Orthogonal partial least squares-discriminant analysis was used to determine important group differentiating metabolites. A: combined PCA for all samples demonstrating distinct clustering by genotype and treatment [sodium citrate (NaC)-treated control vs. streptozotocin (STZ)-treated diabetic mice]. B: PCA differentiating NaC-treated control and STZ-treated diabetic mice within the wild-type (WT) genotype. C: metabolites that differentiated NaC- versus STZ-treated mice within the meprin-β knockout (βKO) genotype. D: metabolites that differentiated the genotypes (WT vs. βKO) within the NaC-treated control group. E: metabolites that differentiated the genotypes (WT vs. βKO) within the STZ-treated diabetic group. Annotated metabolites are shown in Tables 2 and 3.