Table 7.
Impact of genetic prostatic luminal cell ablation on male urinary physiology
| Pbsn4cre/+;R26RTd/+ Mice | Pbsn4cre/+;R26RDta/+ Mice | P Value | |
|---|---|---|---|
| Gross assessment | |||
| n | 8 | 4 | |
| Body mass, g | 25.58 (SD 1.67) | 24.35 (SD 1.6) | 0.1414 |
| Bladder | |||
| Mass, % body mass) | 0.15 (SD 0.03) | 0.14 (SD 0) | 0.5442 |
| Volume, mm3/g body mass | 35.52 (SD 17.42) | 36.98 (SD 20.22) | 0.8992 |
| Prostate mass, % body mass | 0.22 (SD 0.06) | 0.20 (SD 0.02) | 0.3434 |
| Anterior mass, % body mass | 0.11 (SD 0) | 0.12 (SD 0.02) | 0.4629 |
| Ventral mass, % body mass | 0.05 (SD 0.03) | 0.06 (SD 0) | 0.6707 |
| Dorsal mass, % body mass | 0.05 (SD 0) | 0.03 (SD 0) | 0.0222* |
| Lateral mass, % body mass | 0.01 (SD 0) | 0.00 (SD 0) | 0.0242* |
| Seminal vesicle mass, % body mass | 0.76 (SD 0.11) | 0.72 (SD 0.06) | 0.5431 |
| Void spot assay | |||
| n | 8 | 4 | |
| Count | 78.10 (SD 85.11) | 73.00 (SD 109.4) | 0.3677 |
| Total area, cm2 | 36.60 (SD 10.04) | 30.60 (SD 213.92) | 0.4060 |
| Percent area in center | 5.47 (SD 6.50) | 13.50 (SD 26.6) | 0.2141 |
| Percent area in corners | 69.40 (SD 18.36) | 56.00 (SD 41) | 0.4413 |
| Spots of a certain size (diameter) | |||
| 0−0.1 cm | 67.30 (SD 74.95) | 51.30 (SD 72.2) | 0.3455 |
| 0.1−0.25 cm | 6.00 (SD 11.37) | 14.00 (SD 27.4) | 0.3939 |
| 0.25−0.5 cm | 1.25 (SD 1.38) | 4.50 (SD 8.34) | 0.8990 |
| 0.5−1 cm | 1.00 (SD 1.07) | 1.25 (SD 1.9) | >0.9999 |
| 1−2 cm | 0 (SD 0) | 0 (SD 0) | >0.9999 |
| 2−3 cm | 0 (SD 0) | 0 (SD 0) | >0.9999 |
| 3−4 cm | 0.13 (SD 0.37) | 0.25 (SD 0.5) | >0.9999 |
| 4+ cm | 2.50 (SD 0.76) | 1.75 (SD 0.5) | 0.1293 |
| Cystometry | |||
| n | 8 | 4 | |
| Void duration | 0.44 (SD 0.17) | 0.44 (SD 0.14) | 0.9972 |
| Intervoid interval | 5.32 (SD 2.26) | 3.76 (SD 1.74) | 0.2546 |
| Baseline pressure | 2.44 (SD 0.74) | 2.34 (SD 1.24) | 0.7471 |
| Normalized threshold pressure | 5.68 (SD 4.50) | 3.65 (SD 1.54) | 0.4083 |
| Normalized peak void pressure | 19.70 (SD 9.93) | 16.70 (SD 9.6) | 0.6277 |
| Nonvoiding contractions | 6.60 (SD 5.83) | 2.12 (SD 1.14) | 0.0695† |
| Voided volume, ×10−2 | 6.97 (SD 3.08) | 6.14 (SD 2.48) | 0.6720 |
| Compliance, ×10−2 | 1.81 (SD 0.99) | 1.59 (SD 0.90) | 0.7230 |
| Volume flow rate, ×10−3 | 3.69 (SD 2.88) | 2.21 (SD 0.43) | 0.3574 |
| Mass-based flow rate | 31.70 (SD 7.04) | 19.90 (SD 13.26) | 0.0672† |
| Efficiency, % | 107.00 (SD 23.22) | 114.00 (SD 25.6) | 0.6029 |
Values are averages (SD); n, number of animals.
*
Statistically significant difference.
†
Changes that approached significance (trend).