Fig. 6.

Protease-activated receptor-1 (PAR-1) inhibition blocks the signature T helper (Th)17 cell culture supernatant response. Th17 cell culture supernatant treatment of podocytes significantly increased phosphorylation of JNK [phospho-JNK (p-JNK); A], VASP S157 [phospho-VASP (p-VASP) 157; B], p38 MAPK [phospho-p38 MAPK (p-p38 MAPK); C], and paxillin S178 [phospho-paxillin (p-paxillin) S178; D]. Conversely, inhibition of PAR-1 by FR171113 significantly reduced each Th17 response (densitometry based on four blots, by one-way ANOVA and a post hoc Bonferroni multiple-comparison test). Representative blots of proteins were studied (E). VPX, vorapaxar.