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. 2019 Oct 16;10:2346. doi: 10.3389/fimmu.2019.02346

Figure 8.

Figure 8

CCL1, CCR8, and IL4 expression in the colons of mice treated with M2b macrophage exosomes. (A) The CCL1, CCR8, and IL4 mRNA expression levels in the colons of mice were determined by real-time PCR; the housekeeping gene, GAPDH, was used as an internal reference. (B) Western blotting analysis of CCL1, CCR8, and IL4 protein expression in the colons of mice. (C) CCL1, CCR8, and IL4 expression in colon tissue was detected by immunohistochemistry, and the sum of the integrated optical density (IOD) was analyzed.