Table 1.

Forward and reverse primer pairs and associated probe sequences

Gene	Forward Primer	Reverse Primer	Probe	Labeling
vGluT	GCGTTCGTGGACCTTCTAC	TCAGCCACCCTGTAATGGAA	ATCACAGCCAACCTACTTCAGCGAG	CAL Fluor Gold 540-BHQ1
ChAT	GGACCGCCTGGCTAAGTAC	TCGCGGAGTCCCATAAGG	AGGCGGCGCTCAAGCTTCAGAC	CAL Fluor Red 610-BHQ2
vAChT	GCGTCAGCTGCTTCTTCCT	CAGCAGTGCCGTGTCTATGAG	TTCGCCAGCAACTACTGGGTGTT	Quasar 670-BHQ2
ACHE	GGGCAACATGGGCATGTAC	GGTCACCACCGAAGAATTCAATG	AGGCGCTGGCCATCAAGTGGATAC	Quasar 705-BHQ2

Primer and probe sequences target Cancer borealis acetylcholinesterase (AChE), vesicular acetylcholine transporter (vAChT), choline acetyltransferase (ChAT), and vesicular glutamate transporter (vGluT).