Table 2.
Details of the primers used for PCR amplification of EscN/YscN/HrcN family type III secretion system ATPase (hrcN), chromosomal replication initiator factor (dnaA), DNA topoisomerase (ATP-hydrolyzing) subunit B (gyrB), type I glyceraldehyde-3-phosphate dehydrogenase (gapA), hydroxymethylbilane synthase (hmbs), and pyruvate dehydrogenase (pdg) gene.
| Target Gene | Primers Name | Primer Sequences (5′-3′) | Product Size |
|---|---|---|---|
| hrcN | X-hrcN-F | TCGGCACCATGCTCAAGGT | 846 |
| X-hrcN-F | GTGTAGAACGCGGTGATCGA | ||
| dnaA | dnaA-F | CAGCACGGTGGTGTGGTC | 928 |
| dnaA-R | CCTGGATTCGCATTACACC | ||
| gyrB | GyrB-F2 | GAGGTGATCCTCACCGTGCT | 841 |
| GyrB-R2 | TGATGGCCTTGGCTTCGTTC | ||
| gapA | gapA-F1 | TGGCCATCAATGACCTGCTC | 865 |
| gapA-R1 | TAGCCCCACTCGTTGTCGTA | ||
| pdg | pdg-F | CCACCCACCAGACCAAGAA | 990 |
| pdg-R | CAGGTACATGCCCTTGATGA | ||
| hmbs | Hmbs-F | GTATCGCCACCCGCAAAA | 873 |
| Hmbs-R | CCTTGTCGAACAGCCCTTG | ||
| Hmbs-F2 | TTGCATCGCCACCCGCAAGA | 837 | |
| Hmbs-R2 | TCCTTGTCGAACAGGCCTTG | ||
| Hmbs-F10 | AGGGCCTGTTTTTGAAGGAA | 595 | |
| Hmbs-R10 | AACCCCTCGCCTTCCCAGGT |