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. 2019 Oct 18;9(10):625. doi: 10.3390/biom9100625

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Interaction of 5-FAM-KR₉C with giant unilamellar vesicles (GUVs) composed of DOPC/DOPG and DOPC/CHOL/DOPG. (a) Representative images of a single DOPC/CHOL/DOPG GUV at the indicated times after the outside fluoresce reached a constant value (2.5 µM peptide). Top panels: Rho-PE, bottom panels: 5-Figure. (b) Γ_rim = ratio of the fluorescence at the GUV’s rim and at regions outside the GUV after addition of 2.5 µM peptide for membranes of DOPC/DOPG. (c) Γ_rim after addition of 2.5 µM peptide for DOPC/CHOL/DOPG membranes. (b,c) correspond to the data of a single GUV in a representative experiment.