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. 2019 Oct 9;7(10):425. doi: 10.3390/microorganisms7100425

Figure 3.

Figure 3

Schematic diagram of integrating BSMV-γb vector and Osa-miR528 precursor harboring Fg-sRNA1 or STTM sequences. Modified BSMV-γb vector (pCaBS-γ-LIC) was shown in this figure. Osa-miR528 precursor harboring Fg-sRNA1 or STTM sequences can be cloned into pCaBS-γ-LIC derivatives by the LIC reaction. OE-FgsRNA1 structure contained an Osa-miR528 precursor backbone, but Osa-miR528 and Osa-miR528* were changed to Fg-sRNA1 and Fg-sRNA1*. STTM-FgsRNA1 structure contained two tandem target mimics separated by a 48 nt imperfect stem-loop linker.