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. 2019 Oct 9;7(10):425. doi: 10.3390/microorganisms7100425

Figure 4.

Figure 4

Detection of relative transcript level of Fg-sRNA1 and TaCEBiP from F. graminearum infected lesion area in BSMV pre-inoculated wheat leaves. (A) BSMV-based experiment procedures can be found in Materials and Methods section. After 15th day post BSMV constructs inoculation of wheat plants, segments of the 4th leaves of BSMV-infected (BSMV: EV&BSMV: OE-FgsRNA1) wheat plants were collected and then inoculated with F. graminearum (strain PH-1) by the punch inoculation method. Relative transcript levels of Fg-sRNA1 and TaCEBiP were detected by stem-loop RT-PCR and real-time qPCR assay at 1 dpi. Error bars represented standard error (SE) of three representing experiments from four replicates; and (B) similar to BSMV: OE-FgsRNA1infection and punch inoculation assay, BSMV-infected (BSMV: EV&BSMV: STTM-FgsRNA1) wheat leaves were collected and then inoculated with F. graminearum by the punch inoculation method. Relative transcript levels of Fg-sRNA1 were detected by stem-loop RT-PCR at 1, 3, 5 dpi, respectively. Error bars represented standard error (SE) of three representing experiments from four replicates.