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. 2019 Oct 9;7(10):425. doi: 10.3390/microorganisms7100425

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Suppression of YFP–tagged TaCEBiP co-expressed with Fg-sRNA1. (A) Co-expression of YFP-TaCEBiP or its synonymously mutated version (YFP-TaCEBiP-m) with Fg-sRNA1 were observed with confocal microscopy; and (B) Expression of the YFP sensors carrying a Fg-sRNA1 target site of TaCEBiP or a Fg-sRNA1 target site-m, were analyzed after co-expression of Fg-sRNA1. Samples were examined at 48 h after transiently Agrobacterium-mediated transformation into N. benthamiana leaves. (Top) YFP. (Bottom) YFP/bright field overlay. Scale bar is 50 μm. Similar results were obtained in three biological replicates.