Figure 2.

Collagenase production from V. parahaemolyticus and V. alginolyticus in different culture media. The collagenolytic activity was measured using bovine insoluble collagen as substrate. Collagen was incubated with supernatants from liquid cultures of V. parahaemolyticus and V. alginolyticus, grown overnight in the specified liquid media. (A) Collagenase activity measured by colorimetric test. (B) Collagenolytic activity normalized with cell density. (C,D) Trend of collagenolytic activity with cell density in V. parahaemolyticus (C) and V. alginolyticus (D).