Table 1.
Characterization of anti-HA serum obtained after the fourth immunization by competitive and non-competitive ELISA.
| Coating Antigen | ||||
|---|---|---|---|---|
| Antibody | Hapten B-OVA | HA-245-OVA | ||
| Titerb (×103) | Inhibitionc (%) | Titer (×103) | Inhibition (%) | |
| anti-Hapten B#1 | 4 | — | <1 | — |
| anti-Hapten B#2 | 2 | — | <1 | — |
| anti-Hapten B#3 | 4 | — | <1 | — |
| anti-HA-245#1a | 16 | 89.8 | 128 | 26.6 |
| anti-HA-245#2 | 16 | 95.1 | 128 | 37.5 |
| anti-HA-245#3 | 32 | 92.3 | 256 | 31.8 |
a anti-HA-245#1 represents the antiserum from NO.1 mice immunized by immunogen derived from hapten HA-245. b Titer is defined as dilution factor of antiserum with the absorbance at 450 nm being situated at about 1.0 at coating concentration of 1.0 mg/L. c Percentage inhibition was expressed as follow: inhibition (%) = [1 − (B/B0)] × 100. B0 was mean absorbance of the wells in the absence of HA; B was mean absorbance of the wells in the presence of 50 mg/L of HA.