Figure 1.

Plant-derived miRNAs in the larval hemolymph of P. xylostella. (A) Comparison of small RNA (sRNA) reads from the two replicates of GC strain libraries including common and specific reads. (B) and (C). sRNA classification (B) and annotation (C) for the two replicates of the GC strain libraries. (D) Length distribution of plant-derived miRNAs. (E) First base bias at 5′-end of plant-derived miRNAs. (F) Quantitative real-time polymerase chain reaction (qRT-PCR) validation of miR159a, miR166a-3p and novel-7703-5p in the hemolymph of the 4^th instar larvae of the G88 and GC strains. Data were presented as mean ± SD (Student’s t test, * p < 0.05, *** p < 0.001).