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. 2019 Oct 12;9(10):602. doi: 10.3390/biom9100602

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Determination of interaction of miRNAs and target binding sites. (A–C). Luciferase assays of miR159a agomir (A), miR166a-3p agomir (B) and novel-7703-5p agomir (C), together with the wild-type (WT) and mutant (Mut) binding sites. Agomir-NC was used as the negative control miRNA. The mean of the relative luciferase activities of the control group was set to 1. Data were presented as mean ± SD (one-way Analysis of Variance (ANOVA) followed by Tukey’s multiple comparisons, different letters above the columns indicated significant difference at p < 0.05).