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. 2019 Oct 8;9(10):589. doi: 10.3390/biom9100589

Figure 5.

Figure 5

Melatonin production in the M2H RNAi rice seedlings. (a) Melatonin level. (b) RT-PCR analyses of the expression of melatonin biosynthetic genes. (c) Hydrogen peroxide level in shoot. (d) Hydrogen peroxide level in root. (e) Shoot melatonin content in seven-day-old seedlings pretreated with 100 μM melatonin for 24 h. (f) c3OHM content in seven-day-old seedlings pretreated with 100 μM melatonin for 24 h. (g) Proposed model of melatonin regulation by suppression of 2OHM. Rice seedlings grown for seven days in MS medium were treated with 100 μM melatonin for 24 h, followed by HPLC analyses. FW, fresh weight. * Significant differences as determined by Tukey’s post hoc HSD test at p < 0.05.